{ "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "acaba5a0-c174-4bcb-80d9-ea1bcc4187ed", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1305951, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4d5d6dbd-3710-41fb-b62c-bf1d53c94593", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1306230, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a44217d1-a775-46f7-9523-2d206151d9e7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1306512, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5adcda0f-96f2-4a0c-bee2-272355d22abd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1306792, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8ba53705-7b1c-4459-a21a-01effa1684bb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1307070, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7b77a810-004c-45af-9f73-5ed21146c3e3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1307349, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e8f7228e-791b-4807-83e1-a4a9b95e9e55", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1307628, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "07a135bb-bce2-4ab1-853b-4cdf9a5a50db", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1307906, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d85fd24c-b67c-486e-bfef-6549c4ad8307", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1308186, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9c47094-dfdf-4e33-ab85-17510e4f1822", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1308467, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6404fe65-6857-475f-9db9-108351724b4b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1308745, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "350462cd-5765-4043-baf2-eab50bc49952", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1309023, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a1ae405f-b58e-4e2e-b2e2-6c0ef7228fd8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1309301, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "da0593cf-7a47-4234-986f-5f2b36010e11", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1309581, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "795a1907-aacc-428e-9d47-b58c157de687", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1309858, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "88a2c3f1-789d-4055-aab2-f2d5e96ae7f1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1310137, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "980e2a3f-f036-4d7c-9a2c-ca785bdfd156", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1310415, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bd93a601-3186-45bb-8d20-0149add1e169", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1310692, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e3d804a0-f024-407c-9674-be7592110034", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1310969, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "be94e3a2-0ea1-4a42-9656-bb995943cb74", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1311249, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7d4d3fba-b825-4f99-88c3-105c11dc226d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1311527, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d94b58f6-bf9a-4fdb-b1ed-9e32f5e7790d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1311805, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "65cb1b16-d3f4-4108-963f-6eb792702071", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1312085, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "87e013c5-0410-4c72-92a4-d33003900520", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1312362, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bcb68e5c-4648-498f-b9f5-26dff7a249e6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1312641, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0edbdb23-2aa5-424f-a816-df750fe775ac", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1312922, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:44 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "988c78df-68f5-4cbc-96f5-fdb2eb0ae1e8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1313201, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d0ae0da3-1752-4193-9f86-86bde6950d24", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1313479, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d4aa7488-da4f-4943-a899-bdbd3645a2bb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1313757, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:45 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "39b711e3-abb6-43d9-9568-b588f216680b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1314034, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:46 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "18e06c88-a70c-4c6c-b70c-d6ca2952fd33", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1314324, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:46 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "85fd84c7-8576-493c-ae31-7786fd68f6de", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1315243, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "14ac3ab6-0863-4bc0-bf70-f8794a41238c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1315412, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7be0620d-4053-493e-8729-4d6964fa2f89", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1315587, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "64a3fea9-2ece-48ba-900c-bd2612838f5a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1315756, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "23709c84-5c9f-4d6b-91ae-95c1093ce184", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1315926, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:47 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "61963ea4-3714-44ad-b503-3f73f682c89e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1316094, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "673c6982-22c1-49de-83c1-caebe4e40526", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1316261, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9c0ccfe0-7c78-4131-a5d8-8d1944126e3e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1316428, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7101c3bc-3fb1-48f1-a426-a650b0418f45", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1316597, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0a6af4c0-1e16-4bab-8c2f-dbba686a6898", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1316764, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "10537ba7-5e6c-45db-a481-c347def1aa5a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1316934, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:48 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e05dfa4d-c94f-477d-a491-6dd9d80ee5b4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1317104, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1cf4a74c-4615-4cbe-af07-5bd84dad749a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1317274, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dacedb57-7bc8-4326-be03-774a00b1d275", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1317443, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ed81ec23-a58a-4535-bd6a-845c1ace6da4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1317612, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e6c803e1-f416-4e00-a6bf-30d075405d57", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1317779, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "129f027e-da7d-433e-8f0c-11400d24762b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1317947, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:49 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "279d2760-4480-4866-a617-6f87905f0975", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1318113, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "83a023cd-11ba-44bc-9ccd-9e6c3de3ee33", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1318280, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1d999ab1-d650-4549-ba9a-46ea2c4ce99a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1318451, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "27c79684-2259-4165-a76e-50a54a64a989", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1318621, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dee603c8-06d4-446c-961e-21983be29651", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1318789, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c334f2d8-336b-4f76-b5ad-bc8366b06075", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1318962, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:50 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "649f62fa-fe3a-4814-90d5-64b20bc6458a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1319129, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6d47b181-6815-40bc-8428-fbc8e68cd1bb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1319301, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0553072c-ac11-4fe3-bb14-1e47de84292a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1319472, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "16a80d16-40d3-4900-8d81-60a85cec3c08", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1319639, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7fc3991e-0771-4a3e-9ab6-3f200d45faab", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1319808, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ec768b88-f18f-4383-af18-b190dbeb818c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1319978, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:51 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dbc383c5-e3e4-4413-88f8-563b0c8b4e54", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1320149, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:52 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5b9ab1c1-d6bd-4928-a180-fa6a35fbfc1d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1320320, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:52 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5cfbbefc-ebe6-439c-aa80-df2b0390cc44", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1321298, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f4061b50-8746-4e2e-b83f-bf25144a7516", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1321572, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9f87400c-ab2e-4610-bff7-8d15ca7d747d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1321843, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:53 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8270ba1e-d64a-4b84-bcda-38f04d395688", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1322116, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "25367c52-6393-40b1-b119-37f93eec81e6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1322384, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "133044ed-1fa1-461c-a62c-d8b803b78b40", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1322652, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "277d831d-9236-4333-acaa-e3f72b1f9b01", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1322919, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eda9bbae-7853-4708-a95f-7db3320a9aa3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1323190, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b2c79e8d-13d2-4369-980c-c1da0d98374e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1323457, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4454f430-325a-4d71-a5b9-bdbd28b0b84f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1323727, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1b59e7e9-8252-4dbd-b4e5-9da948aebea3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1324001, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2e74d54c-2b02-4828-bc76-9742c3db9355", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1324424, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "146634e0-8ca5-4bd8-9b78-2d21ec9c585c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1324698, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cbfd3cb3-3548-4ef2-a89c-7188fb01c387", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1324967, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "547d0944-3663-45ef-93d0-03b0473de4a8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1325237, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ff4a7a53-d33d-4946-b9c0-fb338d63c89b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1325507, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "afb1dfaa-780a-465e-a5b3-cb3d7a8fb807", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1325774, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9025ac4a-95d7-474a-8ec3-4e69fa18b6d9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1326042, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6af2185a-f63b-4d4b-9800-d98969f57ddd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1326315, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3e4cfe81-ce21-4cda-bf7e-7db49b7795ed", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1326584, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dd91ea8f-5a90-400d-8fc1-3bc597e7129e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1326853, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "337fa3a5-312f-4ef8-935a-686427882fac", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1327122, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "00c76fc0-2acb-47f7-b4a8-09c4f92d5b33", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1327390, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "10337833-8d93-4f8d-b90d-8283e3ecf47c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1327659, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6d5c65bc-3913-40ca-bd22-25267a038e7f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1327927, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:06:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d0a67027-2e3f-48ac-a204-8c8040910c1c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1328195, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9615fc4f-07d7-4815-82b2-7ad6ee9b2117", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1328463, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4489a1a1-149a-4889-8a8f-902d38441f85", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1328730, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "52ea3f42-e07c-4aed-8b52-49cd22c52073", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1329000, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4adcde83-f0cf-4607-a572-272c07f0a2d1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1329270, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a792628a-ee61-4357-8ff8-1ae590d9c075", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1329544, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "35f4341e-f4b0-4be6-8373-66779ce61766", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1330178, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "33a450f9-22e8-4fe9-8d2e-2cef67c573b7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1330525, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a8677926-7e0c-41a6-9fa0-a3050d28093d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1330873, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "49fff9de-1680-478b-a298-8a038dda5852", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1331223, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a2666418-748b-4d9c-8d11-b91f8b2fa560", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1331571, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0064a91f-6007-4774-b95f-cc15df102f52", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1331928, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bc734dc8-c03d-41b2-83ca-1cb319d9c736", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1332276, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7e543275-e4fb-46e2-84cc-d746cb42a894", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1332630, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ced4b573-a190-42ab-a94b-b7b80680b3bf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1333001, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3d6bf9cb-2ce2-4d28-a1ba-318dfaabd454", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1333363, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d156423b-fcdd-4a62-a098-83a9b47269bf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1333712, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "655d6765-95d4-4c40-b7aa-8d52de029c3a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1334068, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f532969d-9013-4df5-a39e-ec33e56d6354", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1334429, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "98afbe64-bf7d-4c66-bd46-8848a6992207", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1334777, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "de7437b1-b5ec-4a36-a564-778345ac5b43", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1335141, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "144152b1-f214-40f3-8074-0672cf659271", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1335489, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "27b877ed-2864-4e5f-aebf-871fde216078", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1335838, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "591ba4c4-64c1-42c8-a0b1-0c318443afd7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1336189, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "15e4131b-eb84-4480-a48e-f1ecca13b3f4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1336554, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6af6a332-39df-47e2-9d08-da1e7a60e4d2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1336914, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5cd51bb2-f242-497a-8750-e4c4f4cbe53e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1337262, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a9df0268-c526-4e68-9920-bd8a1440358f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1337635, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f9a92e0a-369c-45c1-8636-f9928ab5d66b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1337984, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "914124e8-387a-460d-8709-e31d283de771", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1338332, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a657e2f3-7b91-493d-8a01-053a8ea34991", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1338692, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "838947a6-baf4-4c06-9f1d-1f3e8fa4c499", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1339041, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7211c773-691f-426f-aa51-f7d376444890", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1339388, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1a7dd8d6-306a-4af0-aa82-eb4fab7b0ee7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1339736, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "14229a4a-b30f-45d7-a30c-7557f779b03a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1340096, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "73cafb42-3908-443e-b659-075552ad2c6c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1340444, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7be1a698-4c89-49f2-9637-60c05974a753", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1340793, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "07ad27e1-5f7e-434d-bd39-a81a1264861c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1341905, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4da4640c-2a7b-42a6-abf2-fff6240096ae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1342340, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ff361e40-7ae8-44a9-9caf-fbd20ea880ff", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1342787, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "363f6944-f8df-4faa-b1d3-0c3fb6a75f7a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1343219, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6f981550-d82d-4607-b7f0-1483a5215184", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1343652, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0ba7a7f7-905a-4eb9-8752-a30d5fbc7485", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1344085, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9d1ea13d-16d2-4995-acff-dade40416f40", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1344531, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "758f6ff1-cae8-4a74-ae93-76b914d44bfb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1344945, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ead16ca7-9d86-4d14-a9d7-49ea01df4cbf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1345378, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "73a8944b-7e4a-47ad-978f-aa5ebf7a94dc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1345795, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a70d50d1-b593-4a5f-8d44-c52de91a8013", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1346211, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4d405865-5af5-44ed-9c11-d4dcf1bb2438", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1346658, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0089b446-eff5-4c0b-b9ac-9b9fff3bb92b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1347090, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8b56416b-e3d8-416b-a63b-2895cc27ee0d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1347522, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9bb95456-630e-4c19-875e-eef88122e6e5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1347954, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fa44b75a-c040-4556-89d8-f5a1cb7e539b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1348387, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4d32b4ea-053c-48e8-812b-27b1f92bb883", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1348835, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8e80f1d7-35ac-4a73-8e86-ae2460dedd04", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1349249, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "757badb9-3642-42df-94b7-a1d730a77fb7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1349681, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1dbabf95-be61-4d70-b6d3-0a54bdb80381", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1350130, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "267fd812-9d1a-4f4f-96c4-ce771e9d31a2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1350577, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eefb910d-20f8-4e1f-8c7e-aa7887e9ba35", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1351010, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "272c830d-0772-437a-a89e-567aabc5ec55", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1351458, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "20cd23ba-8ed0-4dad-8198-23160e4f3045", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1351904, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2e1de041-3f0a-4532-9c29-3d69162bbb83", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1352353, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f74788cb-369b-402c-9ede-56a9aabafc11", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1352800, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9e5f8ba7-b158-4a39-ae50-46277328bec6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1353222, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b628bbc5-84fd-4d8f-91c1-c703b92e517e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1353649, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f6e7453e-e02e-4278-8ddf-1cc4f7fd1cf0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1354081, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "54352c14-7b04-4279-bd4d-c0e2bf9a2bb4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1354513, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fadc7620-1e4c-4dc6-a92c-cb8d76923e60", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 26, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 19:06:37 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 26, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1354959, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_005_003", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 62690.056, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 19:07:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 204.528, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }