{ "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "52ffcf4f-50ce-47ae-a322-6335e76747e8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": 0, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2265, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e6271c6b-0165-4fbe-aa5d-5db7b5d7f276", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2729, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5c59b305-bf2a-40de-9a85-20a2756f4ea3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3006, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:54 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ed024905-a0af-448c-a8f5-aebb4b02fee2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3286, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "02c8d001-1c17-4a7a-a9f4-656b616738af", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3565, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7707c451-285a-4787-831b-e6b566de85a7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 3869, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:55 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1e874460-84fd-46c6-9624-2cdd4f58bd5a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 4148, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef74a02d-b7d8-4983-af24-19deef43f3d6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 4426, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "78ef5928-e8ae-4aff-88fa-ffbeaf1ab5cd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 4719, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "53c3b861-0397-4fc0-89df-8c9e4b41b1de", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 4997, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:56 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "301e3c2b-95c7-429b-9dd4-c96d7f98fcc2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 5302, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "21cbd379-68f9-4fc5-8a3e-fccba2213028", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 5581, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4bc87e93-f574-4963-9d4f-cfd95fa6ec78", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 5861, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:57 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ce890e02-a9f1-4bad-bf79-c16ff56d1f21", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6140, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "980d359d-ea27-4826-aa26-901a4fe9e238", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6419, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "da753af5-4ffb-4f35-bccc-4a813652928d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6711, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0e5d078b-bb74-4219-9ae6-74e7866dc476", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 6989, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:58 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cabe8fa1-c196-4b51-812f-2110b99e1c59", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 7266, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "804a4fa7-db0c-49b5-bd84-b9eabbcacf35", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 7543, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7f7bade1-b00d-4aa5-93d7-a3b04f358a43", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 7820, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:44:59 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "98df6a6a-2cf4-4efa-a3b4-f51d8c58b1ba", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 8100, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d6fad6ff-a9d9-4c22-a6d3-892e12c61cf2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 8378, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "01be2f71-29b4-454b-86da-8b366f2dcdb5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 8657, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "44e499d0-cc4b-407f-8fc5-031d60497476", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 8941, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:00 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "67b12676-3e8a-4d95-b741-d747b0c21c58", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 9238, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "34868174-b891-45ec-80e7-c8570466d2d5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 9516, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "18a61b51-9b5c-445c-9a6a-6bcf2862b6ee", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 9794, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:01 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ee55a454-92d7-4971-83e9-288c0e483ddf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 10074, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5933153c-6f2e-414b-a3e6-32fdadd27ea4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 10357, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "df522205-20c7-43b4-b6fa-8697a06a2aeb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 10659, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 190, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a22833e5-f993-47c0-a84a-dec42d31ae50", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 10937, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "190.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:02 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fe338e21-ec0e-4af1-8140-bca3f1e26d50", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 11860, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:03 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd9c293f-2971-46c3-a47e-a5f9b408a401", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 12030, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d06b84cd-5112-4b73-b27a-9201dcbff5ca", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 12206, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "72b51e70-0883-4f4a-90af-74e78e920e0f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 12403, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0841d25f-a6de-41b2-aef2-14ef0f590cf7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 12572, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1f9ad8cb-2fc7-4732-b09e-dd19a26ebb68", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 12742, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "11044b28-69f3-4dd7-8fb0-44b75be34be4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 12909, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:04 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6a3b0cfe-034d-4414-bb08-5b94fe399b05", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 13079, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c576b3bc-aa59-4f5f-bf44-51a775db827e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 13258, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b38f53ca-4a47-42c7-87c5-a9259397ad92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 13427, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "58d97df6-8d97-4546-a932-04e2db8ed281", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 13595, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "595d3a9f-337b-4709-9eda-63edc6c768e6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 13763, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2b20fb9b-ba93-4ee5-a495-4baddb3670f5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 13935, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:05 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c7c110f4-9520-481f-b073-cee426a05479", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 14116, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1eaacf68-c642-4aa1-b727-ffb1adcd615d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 14306, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0725ebd4-cdf5-487c-aaa7-e524e0da8501", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 14474, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7edbb40b-795f-4a44-8aaa-c778709cd85d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 14641, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6e7d5dc7-3030-4e10-bb36-85fe0581e620", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 14810, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "85bba284-26d8-4f18-a079-7f7a2d81e1f4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 14978, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:06 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b4f5c638-8231-445b-9e4d-6e867062af34", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 15157, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aa845914-ea33-4491-b271-4f0c37ae07de", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 15328, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c2b02c78-16b7-48df-ba03-8f9783b3ad5c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 15496, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e426b200-f6d3-4eb4-a76a-a47a9115f45e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 15664, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "60ba6af4-f758-4b87-a88a-6d7c88bdf94f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 15830, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b11ef8fc-0e5c-41a2-a5e9-f6e27f7bd4af", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 15999, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:07 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dad5af24-ea36-41c5-b467-d046346575fa", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 16173, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e1826532-fac7-4ac2-9195-1d92d24af58a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 16357, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c0a3d0b3-7d01-410b-b9c0-4bbf28b31679", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 16526, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cf090cac-e7dc-4813-a7ce-746d231e4782", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 16696, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3d66258b-1c75-4976-aed7-f0d51c979562", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 16866, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:08 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 80, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d8e49a9e-3ee3-4e9c-9aa0-4a87cdae62fd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 17035, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "80.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d2fdc270-13a3-4e81-9886-73c3e8a2054c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 18005, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:09 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "65f4cd8f-1fdf-46ff-b18e-c11fd4086690", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 18275, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ddcbc556-f458-4c2e-9bea-f00089836a87", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 18543, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8d479036-fe7b-4fcb-9ad1-0facbaa27b19", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 18813, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:10 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0f209325-faad-44d1-99d0-656ec22fd795", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 19091, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1f9072d7-4bc0-4e2d-9380-10460b6a5aac", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 19383, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "97604486-d93a-4852-809f-95f549428a25", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 19655, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "18eb7270-3fc3-4fea-86a9-ecea2b02e54a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 19924, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:11 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a2496fa9-10f5-4f54-b1c8-1db9df2799f3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 20192, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8f91edce-275c-4dfc-8a19-babc2a449a70", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 20461, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8e0f9c9a-1763-41db-8c77-1af9216798f0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 20732, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2a1bd5cd-df95-4735-aeb1-123de6bd2908", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 21003, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:12 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7141ae7e-c855-4be8-84e5-6124126f8634", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 21271, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "34a8714f-f753-4f54-bce7-6c75b905d24f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 21540, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e231761b-0125-4f7b-bc25-7ec9229d0b7e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 21809, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:13 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2d6b4300-b274-454a-9e93-7bf25ad66645", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 22082, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "deed5a40-929a-4845-8e44-71f7342deb6b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 22354, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "aecc83cc-9805-42a5-9912-7f0cd66ec8f9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 22623, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "325560a3-8aa9-47be-9aab-081d31e3f021", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 22893, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:14 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a88cd5b5-9d1b-4bce-82f3-8d29516b1bcf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 23160, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0eb6f887-4562-4dbf-b3be-4e38f329c671", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 23437, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3ed6b4dd-8d05-4454-b5c0-2eb81dc883c7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 23710, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bdcc1666-bd5c-4075-ba5b-e62e49217426", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 23980, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:15 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8592926f-f2a9-4aae-9c67-d9b94c38e8fe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 24247, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0d6a3793-7ab7-42b2-a7a5-2db673875d76", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 24515, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6e8885a5-6996-41cc-a6bd-78c643759d1a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 24782, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:16 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0f00cf32-e4b6-41a6-a381-c01ef2abc8a3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 25051, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "08ee5297-ef62-49f0-8d64-6bdf3b493ccb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 25321, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6ffc50a4-d870-4133-8d16-1ce8a7a74f3f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 25591, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4f24d90a-e2ff-48b1-bdf0-2a28cc77bb64", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 25862, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:17 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 180, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e6ce9135-5cb9-4a32-94c4-93ff9a66992c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 26136, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "180.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0e1258a3-81ac-4755-8faf-7ae708fd292b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 26761, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:18 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "afaf49fa-4ca6-4a37-9d04-99dc5db4628a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 27109, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "46071f1b-c3fe-4ff6-b46a-85bf1f0cf895", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 27458, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5b163c8b-44a9-40ba-8229-37c40dd4b7f2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 27811, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:19 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2c5e9f5b-8030-4aa3-bf71-a3b86864e9ec", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 28174, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c09e7891-18c0-4e5f-9a42-991b02310935", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 28535, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a3182dc9-3af4-495f-9a6c-433530dfaea7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 28889, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:20 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fe408891-cbd8-48b8-9430-b8e9131f0d18", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 29243, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9bea69ff-3fb4-4416-bdd3-8c851df2e794", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 29599, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e47b0f9a-133c-45fd-bb4c-a87bb28d6603", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 29952, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:21 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6857fba4-163b-4e0a-bac2-0af9328795a7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 30309, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7addea0f-1905-45b5-9f4a-ebbc6311d6d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 30660, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2ab4972b-d1bb-4169-a4a5-7c887bad8bd4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 31009, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:22 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d9ee247b-bf19-40b0-a925-adacde6bfd89", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 31359, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1a315f36-46e7-4d25-926c-a914c7378f23", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 31709, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:23 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b40c1784-853d-46b9-bfab-86e9e8e59f61", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 32059, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eb15e1c6-8d3f-4087-8d65-3e892783e597", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 32408, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d139a6b1-25c9-4d72-91b6-3b708f1f3bd4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 32759, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:24 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ee08324d-ad9e-45ce-86c5-7119c6b95538", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 33109, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2b5f8192-8aab-473d-b79d-d9a0841b951c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 33458, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8764627e-f029-48a1-b5df-1cfa3e57aed9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 33805, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:25 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dd087a65-8606-4243-9dd7-a04545c11979", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 34156, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c0bffd43-af3e-40d7-bb38-04b5ad0c770d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 34507, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b0a1ffc9-5005-45e2-a88a-f944de46f5b6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 34854, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:26 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "12ce6d84-e74e-4de9-b101-9251422c3622", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 35205, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f25d5cfd-bfa2-4104-844b-5e13461a5235", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 35555, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3199786e-07a4-4e82-a719-9a1767c638ec", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 35905, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:27 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "028257e9-6048-4cca-8682-6a3609c53c32", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 36251, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:28 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "947ac3dc-eca5-4fed-9a1c-38f915eeae7f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 36601, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:28 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e4e09865-3b3d-4e1b-b22c-7c32dcac5303", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 36949, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:28 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 260, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "69970f65-4863-43d6-81ee-ec604c8ca8c0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 37306, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "260.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:29 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a323a19f-005f-40cb-9021-60df38994d41", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 38424, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:30 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2687affc-60d4-4a20-82d2-52cb1b3b929b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 38855, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:30 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "384eb83f-db60-4ea0-b17b-4996514d2d94", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 39303, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:31 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "24beb99f-5644-4e4c-a4a9-837be0f80cf8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 39751, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:31 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fdc1e313-37be-42f7-9191-f74ed5da1bff", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 40183, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:32 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "af4cf5a6-8c73-41dc-b841-985052bea26c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 40631, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:32 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a84055ca-e01a-4287-9ffd-8ab542444cf6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 41064, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:33 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "265c7d41-eee3-4dd8-9588-b79f0607c0b9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 41511, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:33 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "92a22122-8b83-4666-867d-f71d15b445c7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 41959, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:33 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd0ff01b-f7e3-44ad-a8a4-ac2d54d82511", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 42374, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:34 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "23be8c4a-53f8-4c26-9274-ab5462a692ad", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 42806, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:34 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3e9af5e0-ff00-462b-bea6-f258812ad70a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 43239, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:35 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1830399a-6443-4ede-a57b-43f07ef4d281", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 43671, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:35 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a0f88ebb-45f6-40a7-9a6c-b9e93f58a5d7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 44118, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:36 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8a8d5e2f-bf3e-4565-8dcb-830973475398", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 44550, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:36 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8fafc970-8a58-4c9c-af82-7b9605e1d2df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 44998, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:36 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8834143b-0299-401a-8bde-36aa209fa6df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 45445, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "41853708-0b8e-499f-a93a-3cdfbc7991f9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 45893, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:37 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8d80a2a1-c7be-429c-bb15-fac8c7d451ce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 46326, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9fde7948-974a-42f2-a469-95a48d43863c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 46758, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:38 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd769589-eace-4c6d-aa08-9c8fab1a1a2c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 47191, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3a633c1d-1fc2-4b8f-a670-b9a7a2a48887", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 47620, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:39 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7ebc4435-dfd3-420b-a7a5-ddd3fa787ce1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 48039, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c5e55ad9-124e-4752-831b-ee07efda13ae", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 48486, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b0c0d924-dd83-4dba-904a-166890173653", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 48917, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:40 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "241f57ce-05f8-4afa-a5e4-af36b348d157", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 49333, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2ee14fda-243d-48b0-8a0d-86630c803121", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 49796, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:41 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a50a84a5-03ca-49d1-882e-5185d0d5e87c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 50229, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b55adda3-fc9a-458c-a373-8b39d8698364", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 50676, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:42 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "60b1d3f8-2d90-405b-ba90-32dfcbff18a1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 51109, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.8500", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a218ae2c-252e-47b5-a997-162dc7cef38d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 0, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -631.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -631.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -631.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -631.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -631.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -631.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -631.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -352.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -352.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -352.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -352.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -352.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -352.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -352.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, -74 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, -74 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, -74 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, -74 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, -74 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, -74 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, -74 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 204.528 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 204.528 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 204.528 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 204.528 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 204.528 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 204.528 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 204.528 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61297.416, 483.056 ] }, "GridColumnIndex": 0, "Label": "M1-L2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61575.944, 483.056 ] }, "GridColumnIndex": 1, "Label": "M1-L2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 61854.472, 483.056 ] }, "GridColumnIndex": 2, "Label": "M1-L2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62133, 483.056 ] }, "GridColumnIndex": 3, "Label": "M1-L2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62411.528, 483.056 ] }, "GridColumnIndex": 4, "Label": "M1-L2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62690.056, 483.056 ] }, "GridColumnIndex": 5, "Label": "M1-L2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.86], "DA Z Stage": [110], "XYStage": [ 62968.584, 483.056 ] }, "GridColumnIndex": 6, "Label": "M1-L2_006_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1508.056 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1508.056 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1508.056 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1508.056 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1508.056 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1508.056 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1508.056 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -1229.528 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -1229.528 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -1229.528 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -1229.528 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -1229.528 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -1229.528 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -1229.528 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -951 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -951 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -951 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -951 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -951 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -951 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -951 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -672.472 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -672.472 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -672.472 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -672.472 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -672.472 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -672.472 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -672.472 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57315.416, -393.944 ] }, "GridColumnIndex": 0, "Label": "M1-R2_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57593.944, -393.944 ] }, "GridColumnIndex": 1, "Label": "M1-R2_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 57872.472, -393.944 ] }, "GridColumnIndex": 2, "Label": "M1-R2_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58151, -393.944 ] }, "GridColumnIndex": 3, "Label": "M1-R2_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58429.528, -393.944 ] }, "GridColumnIndex": 4, "Label": "M1-R2_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58708.056, -393.944 ] }, "GridColumnIndex": 5, "Label": "M1-R2_005_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8437.93], "DA Z Stage": [110], "XYStage": [ 58986.584, -393.944 ] }, "GridColumnIndex": 6, "Label": "M1-R2_006_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "779edfd3-2e99-41d1-ba22-18177506a583", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2020-01-12 18:44:54 -0500", "Date": "2020-01-12", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 0, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq4_L2R2retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 70, "Directory": "F:\\20200111M1genes" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "49996000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "2", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 51523, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "M1-L2_000_000", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 61297.416, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8403.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2020-01-12 18:45:43 -0500", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": -631.056, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "8.0000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }